Assay Procedure

Materials provided

M5 Coated Microstrips

M30 Standard A 0U/L

M30 Standard B, 75 U/L

M30 Standard C, 150 U/L

M30 Standard D, 250 U/L

M30 Standard E, 500 U/L

M30 Standard F, 750 U/L

M30 Standard G, 1000 U/L

M30 Control Low

M30 Control High

M30 HRP Conjugate

M30 HRP Conjugate Dilution Buffer

Wash Tablet

TMB Substrate

Stop Solution

Sealing tape

Instructions for Use

Certificate of analysis

Description

   96 well microplate, 12 strips with 8 wells, Ready for use.

  2.5 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0.6 ml, in see through glas vial. Ready for use.

  0,4 ml, in brown glass vial.

  12 ml, in see through glas vial.

  1 tablet for preparation of 500 ml wash solution.

  22 ml, in brown plastic vial. Ready for use.

  8 ml, sulphuric acid, in see through glas vial.Ready for use.

  1 sheet.

  –

Format

1 plate

1 x 2.5 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.6 mL

1 x 0.4 mL

1 x 12 mL

1 x

1 x 22 mL

1 x 8 mL

1 x

1 x

• Microplate reader (wavelenght:450 nm; linear 0-3 OD)

• Microplate shaker (oscillation: 600 rpm)

• 96 well microtiter plate washer or multichannel pipette (250 µL)

• Vortex mixer

• Precision pipettes: 25, 50, 75 and 200 µL

• Cylinder (500 mL) Deionised water