M65 EpiRat™ ELISA
Product no. 10060
The M65 EpiRat™ ELISA is based on the M5 and M6 antibodies, which bind to the biomarker Keratin-18 (K18). K18 is an intermediate filament protein in epithelial cells.
The concentration of K18 reflects the amount of overall cell death, both apoptosis and necrosis, in K18-positive cells. Levels of K18 are commonly elevated when the liver endures damage, through disease or toxicological effects.
General Information
M65 EpiRat™ ELISA M65 EpiRat™ ELISA M65 EpiRat™ ELISA M65 EpiRat™ ELISA M65 EpiRat™ ELISA
Application Fields
Toxicology
The M65 EpiRat™ ELISA can be used in toxicological studies. Rodent species are commonly used in traditional toxicology testing guidelines to predict human health toxicity outcomes. Rodents have significant conventional knowledge, historical availability, and ease of care and breeding, making them ideal test subjects for the assessment of possible adverse health effects and sensitivities from chemical exposures.
As rodent species are commonly used globally in traditional toxicology testing guidelines to predict human health toxicity outcomes, the M65 EpiRat™ will help by quantifying the amount of cell death in hepatocytes to assess liver toxicity. This in turn saves Pharmaceutical companies a lot of money where they can discontinue projects where the tested compound has too high a toxicological effect on the liver.
Research
The M65 EpiRat™ ELISA detects total amount of K18, which quantifies the total amount of cell death in epithelial cells. This allows for the use of the assay in different research fields such as in hepatology and oncology. In pre clinical studies, the M65 EpiRat™ ELISA can be used to analyse the effect of drugs on treatment effect and disease progression for example for new compounds in Non Alcoholic Fatty Liver (NAFL) and Non Alcoholic Steatohepatitis (NASH).
M65 EpiRat™ ELISA’s unique characteristics
Rat-specific measurement of total cell death in K18-positive cells
Quantification of K18 in rat serum and plasma samples
Convenient ready-to-use format and can be split up for use on several occasions
Sandwich ELISA in a 96-well plate in a convenient ready-to-use format
Materials included:
M6 coated microstrips
M65 conjugate
M65 standard A-E
Wash tablet
TMB substrate
Samples
Rat serum and plasma
The PEVIVA products in Drug Development
M65 EpiRat™ ELISA in:
RESEARCH
Oncology
In oncology, cancer tumor cells prolifirate quickly and do not die as fast. Therefore, causing cell death in cancer is of importance. The VLVbio Keratin 18 assays are valuable in all of the stages in anti-cancer drug development; in drug discovery, drug development, clinical trials, and clinical research.
RESEARCH
Drug-Induced Liver Injury
Drug-Induced Liver Injury (DILI) is one of the most common reason for acute liver failure and a condition that may require liver transplantation and in some cases even has fatal outcomes.
Today, DILI has implications in both the development of new drugs and in clinics due to toxicity of existing drugs on the market.