The latest addition to the PEVIVA product family will be released soon!

M65 EpiRat™ ELISA

The new edition to the PEVIVA product line, M65 EpiRat™ ELISA, is optimized as a rat specific assay and represents the final piece of the puzzle for the complete K18 portfolio, making the PEVIVA product line available and valuable in all stages of drug development.

Figure 1. PEVIVA product line in Drug Development
With the new arrival of the M65 EpiRat™ ELISA, the PEVIVA product line is now available throughout all stages of drug development, from the discovery and pre-clinical phase up until the clinical application of the drug.

Technique

The M65 EpiRat™ ELISA is based on the M5 and M6 antibodies, which bind to the biomarker Keratin-18 (K18). The concentration of K18 reflects the amount of overall cell death, both apoptosis and necrosis, in K18 positive cells. Levels of K18 are commonly elevated when the liver endures damage, through disease or toxicological effects.

Toxicology
The M65 EpiRat™ ELISA can be used in toxicological studies. As rodent species are commonly used globally in traditional toxicology testing guidelines to predict human health toxicity outcomes, the M65 EpiRat™ will help by quantifying the amount of cell death in hepatocytes to assess liver toxicity.

Research
In pre-clinical studies, the M65 EpiRat™ ELISA can be used to analyse the effect of drugs on treatment effect and disease progression for example for new compounds in Non Alcoholic Fatty Liver (NAFL) and Non Alcoholic Steatohepatitis (NASH).

Figure 2.
11 Plasma samples from untreated rats and rats treated with different Paracetamol (APAP) doses were run using the M65 EpiRat™ ELISA. The figure shows an increase in M65 EpiRat™ values in rats treated with 1000 mg/kg APAP and 1500 mg/kg APAP, in comparison to the untreated rats. Blood was drawn after 0, 24 and 48 hours for each rat.

M65 EpiRat™ ELISA’s unique characteristics:

• Rat specific measurement of total cell death in K18 positive cells
• Quantification of K18 in rat serum and plasma samples.
• Convenient ready to use format and can be slit up for use at several occasions

We are very happy to welcome the M65 EpiRat™ ELISA as the newest member of the PEVIVA product line!